Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $ 7,900. Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). The multi-mode microplate reader provides flexibility and ease of use for a variety of applications Market Highlights Microplate Readers Market is predicted to project a notable CAGR of 4. Transfluor Technology. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. The OD 600 was plotted against time using Graphpad Prism 8. Chemiluminescence emission was measured in real time (every 2 min for 120 min) with a FilterMax F5 Multi-Mode Microplate Reader at 27°C and expressed as area under the curve, as previously described . DHE fluorescence was recorded on a Filtermax F5 multi-mode microplate reader. FITC-conjugated casein assay substrate was added to the wells and incubated at room temperature for 2 hours. $3,000 USD. Renilla/Firefly luciferase activities were measured twice. 1 MB Table of Contents. . This document is provided to customers. The following document is. MetaFluor Software. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. Fold changes were calculated as the area under the curve of stimulated HKLs relative to unstimulated HKLs (RPMI without zymosan). 8. Transfluor Technology. Important things to consider when purchasing a multimode microplate reader. 3 Pages. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. The planktonic growth inhibition (%) was calculated according to the following equation:Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. We recommend you read this assay in dual emission mode, detecting both the emission from the Europium donor fluorophore at 615 nm, and the acceptor fluorophore (at 665 nm for APC. The present study found competitive inhibition of hMAO-A by ILG along with a similar mode of action against hMAO-B as in rMAO-B. #ID5-STD), equipped with the following: Enhanced TRF Module (Molecular Devices cat. 4. Instrument Configuration: Assay Technology Adapta® & • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate. Instrument Configuration: Assay Technology Adapta® & F3/F5 Multi-Mode Microplate Readers. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Description: The FilterMax™ F3 features absorbance (visible),. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. 0. 50/50. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 4 Pages. SoftMax® Pro Microplate Data Analysis Software provides integrated instrument control and data analysis. CloneMatrix. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help save time and reduce common errors in the lab—including automated. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. All ELISA kits were read using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices; San Jose, CA, USA), according to the kit guidelines. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument. 4. x. The door opens, revealing the transport lock that fastens the microplate drawer to the internal frame of the instrument. Safety Information. All experiments were performed in triplicate. Read Review (1)The mixture were then centrifuged at 1000 g for 5 min. SpectraMax Quant dsDNA Assay Kits. 425 or. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. 713579). ” e. Dave Zeckhausen. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. A microplate reader (also called sometimes microtiter plate reader) is a high-sensitivity instrument designed to analyze samples contained in microplate wells. LabX is a multi-vendor marketplace that connects you with the life science tools and chemistry you need to run your lab. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. 5 ml 0. 3 Pages. Many absorbance assays use a chromogenic substrate, which, upon enzymatic conversion to the final product, results in a compound thatThen 200 µl of 10% (v/v) ammonium hydroxide was added to neutralize the acid and absorbance readings were taken at 405 nm with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices. View specifications, prices, citations, reviews, and more. 020 fmol 0. Measurements of Caspase-3/7 Activity. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se Figure 1: Schematic of a typical workow of the semi solid cloning process. Create an experiment based on the protocol. SpectraMax Glo Steady-Luc Reporter Assay Kit. 012 fmol 0. A 1:1 binding ratio Hill model was used to fit the binding interaction data. 4) onto the FilterMax F5 to be read with SpectraMax® Paradigm® Multi-Mode Detection Platform. Multi-mode Microplate Readers. 2 Pages. FilterMax F5 Multi-Mode Microplate Reader The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence,. Add. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and. Absorbance mode reads plates up to 0. SoftMax Pro Software, data can be visualized as gray scale, kinetic plots or reaction rates. 8 Pages. Perkin Elmer Wallac Victor2 1420 Multilabel. 4 Pages. Activation of TLR4 and TLR2 receptors. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. This document is provided to customers. GenePix 4300/4400 Microarray Scanner. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. S. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. SpectraMax MiniMax 300 Imaging Cytometer for the SpectraMax i3x Multi-Mode Microplate Detection Platform KEY FEATURES • ombine cellular imaging C and microplate assays into a single workflow • liminate cell staining for cell E counting and confluency with StainFree Technology • omplete cell viability C and toxicity assays with. In . With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. ScanLater Western Blot Detection System. 4 Pages. 3 Pages. ABS Test results. 4 Pages. Firefly and Renilla luciferase relative light units were measured using the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). By category. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. com B. Detection modalities are shown in Table 1-1. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. , Sunnyvale, CA, USA) and. 2 μM ATP standard was. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. 5 μg/ml) in the dark for 1 h. CatchPoint cGMP Detection Assay Kit. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. By category. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. Excitation Filter – EXP1 F535/25, F485/20, F485/20, F370/TRF. 003 fmol 0. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Maximum haemolysis was obtained using ultrapure distilled water, and untreated erythrocytes were used as a negative control [14]. The user must evaluate this instrument and PC-based software in conjunction with their specific assay(s). Zebrafish whole-eye mRNA extraction and RT-PCRSearch LabX. Approximately 50 µl of these. Then, 50 µL of a stop solution were applied to terminate the reaction. CloneMatrix. GPCRs. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. Learn More. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. 3. SpectraMax Glo Steady-Luc Reporter Assay Kit. Multi-mode Microplate Readers. • DNA/RNA quantitation • Reporter gene assay The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. They provide absorbance, fluorescence, glow luminescence, and other read modes for Affordability and unmatched assay performance in filter-based microplate readers. ImageXpress Micro 4 High-Content Imaging System. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Accurate measurements are ensured by automatic lamp calibration prior to each reading. An array of these tubes is situated behind the sodium iodide crystal and may. BioStack 4 offers patented plate de-lidding and re-lidding for sensitive cell-based workflows. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Imaging by immunofluorescence. 7°C (gradient heating design). c. 8 Pages. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumFlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . HTRF-compatible SpectraMax Multi-Mode Readers. The plates were loaded into a FilterMax TM F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA), and the fluorescence intensity was measured at 37 °C with Ex/Em = 440/484 nm and 15 s shaking between reads to facilitate aggregation. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#:. 5 fmol/100 μl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 μl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in. At day 4 P. Homogeneous Time-Resolved Fluorescence (HTRF) . Other technologies such as imaging. I. SpectraMax iD5. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. The OD 595 of the culture in the plate (light path length: ∼6. 2 Pages. Cells were seeded in 24-well plates and cultured. Molecular Devices technical support. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of. The quantity of colorimetric product was continuously monitored for 120 min. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Laser-aided direct metal deposition of metals and alloys. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. 6. Detection cartridge modularity allows for easy addition of new read modes within minutes without. VANTAstar™ Flexible Multi-mode Microplate Reader. 3 and higher include optimized reader settings, as well as data analysis, for faster results. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seEye lysates were developed using a Glucose Colorimetric Assay Kit (Sigma, Louis, MO, USA) and measured by a FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 570 nm. The VICTOR Nivo multimode microplate reader is a high-performance filter-based plate reader equipped with all major detection technologies – Alpha, luminescence, etc. Search LabX. In addition, the FilterMax F5 Reader is capable of measuring. Molecular Devices, LLC Reading Modes: Endpoint, Kinetic, Linear Scan, Area Scan; Optical System: LEDs, Deuterium Lamp; Wavelength Range: 230 to 750 nm;. DCF fluorescence was measured by using a microplate reader (FilterMax F5) at maximum excitation and with emission spectra of 485 nm and 535 nm, respectively. MetaMorph NX. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). The readings of samples incubated with different carbon sources were subtracted from the average of the control readings. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices. BMG LABTECH. 8 Pages. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. the luminescence reading was taken on a FilterMax F5 Multi-Mode. 2. 6 Pages. » FilterMax™ F5 Multi-Mode Microplate Reader » SpectraMax L Microplate Luminometer ATP + Luciferin + 0 2 Oxyluciferin + AMP + PPi + CO 2 + Light Mg2+ Luciferase Benefits • Sensitive, reliable detection of mycoplasma contamination. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. 50/50. SpectraMax Paradigm Multi-Mode Safety Guide; SpectraMax Paradigm Multi-Mode Unpacking Guide; SpectraMax Paradigm Multi-Mode. Add to my manuals. MetaMorph NX. Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set. Z’-LYTE® Compatible Microplate Reader Documentation Version No. 3 Pages. Readers ®Examples: Monochromators such as the SpectraMax i3 Multi-Mode Microplate Reader Detection Platform or filter based systems like the FilterMax™ F3 and F5 Multi-Mode Microplate Readers Luminescence Principle The total amount of light emitted is collected Plate specs White plates to reflect all the light to the detectorSpectraMax i3/i3x Multi-Mode Microplate Reader HeLa cells Cell viability assay SoftMax Pro Software Simplified homogenous experimental protocol The EarlyTox cell viability assay kits apply a no-wash, homogeneous protocol that simplifies the workflow and reduces the data variability typically encountered with multiple experimental steps. F3/F5 Multi-Mode Microplate Readers. After the mixture was cooled, TBARS was measured at 532 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Reader, Molecular Devices). Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. Delete from my manuals. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). Mazumder, in Laser Additive Manufacturing, 2017 1. Coculture experiment. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). Protein contamination can be measured at 280 nm. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. Renilla/Firefly luciferase activities were measured twice. The supernatant was taken and 0. 2 Temperature and cooling rate control. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 3 Pages. 00. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). Compact designs make these instruments. This microplate reader also features BioTek’s Hybrid Technology, which. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. CloneMatrix. The OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. A 1:1 binding ratio Hill model was used to fit the binding interaction data. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Measurement of Caspase 3/7 Activity. i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. 561. 00FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. SoftMax Pro GxP Compliance Software. Page 25: FeaturesThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. FilterMax F3 & F5 Microplate Readers. Caspase-3/7 activity, an indicator of apoptosis, was measured by a luminescence assay using caspase-Glo. 3 Pages. 01% in 2030. This reader provides visible absorbance. SpectraMax Paradigm Multi-Mode Microplate Reader. After 30 min, the reaction was stopped by adding 1 N HCl and absorbance at a wavelength of 490 nm was measured using the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Select the protocol “Concentration_pico_green_v3. LDH cytotoxicity assaySome of new FilterMax F5 Multi-Mode Microplate readers are shipped with ARP USB to serial port adaptor (Manufacturer No: 572213, ARP No: 465338, 1 X USB, 1 X serial DB9/m, blue). ScanLater Western Blot Detection System. Fluorescence kinetics were monitored from the top in a FilterMax F5 Multi-Mode Microplate Reader and the Multi-Mode Analysis Software 3. SpectraMax Glo Steady-Luc Reporter Assay Kit. FilterMax F3 & F5 Multi-Mode Microplate Readers. aureus , the amount of bacteriophage-MBs and the dilution ratio of the HRP. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. Each kit was used on the same day that its reagents were reconstituted. Products : General, FilterMax F5 reader. Yes, the M5 does have a limited slip. Your applications, your modes, your choice. A blank sample was prepared by mixing. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 4 Pages. 003 fmol 0. ScanLater Western Blot Detection System. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. Samples were considered positive if the average sample absorbance was greater than twice the mean absorbance of healthy. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Transfluor Technology. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 2. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. 05); b Evaluation of the ATP levels of T. SpectraMax iD5. To contact technical support by phone in the U. com 8. The reactions were analyzed in a fluorometer (F5 Multi-Mode Microplate Reader) at 590 nm after a 120-h incubation. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Description Z-height focusing is enabled for fast top- and bottom-reads for all read modes. 4 Pages. Neurotransmitter Transporter Uptake Assay Kit. 5 min for 30 min at 340 nm absorbance using a microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, FilterMax F5 Multi-Mode Microplate Reader) and Multi-Mode Analysis Software (Molecular Devices, RRID:SCR_014789), version 3. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. FilterMax F5 Multi-Mode Microplate Reader. S. 5 mP. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. 012 fmol 0. Readings at 575 nm were subtracted from the readings at 450 nm to correct for optical imperfections in the plate. Users can program endpoint, kinetic, multiple wavelength, linear scan, and area scan measurement types. All Synergy HT models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. ”SpectraMax® Paradigm® Multi-Mode Detection Platform. The concentration was expressed as μmol/mg of total protein, based on the use of a standard curve of chloramine T (0-100 μmol/L). Download Table of Contents. Read Review (1)FlexStation® 3 Multi-Mode Microplate Reader. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched value and flexibility for a variety of applications capable of performing: - Absorbance detection in the visible range (340 nm to 650 nm, F3) or UV and visible range (230nm to. 0. Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. 1. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat SYRINGE/VALVE CHAMBER FOR RQF INSTRUMENT quench flow analysis KIN-TEK RAPID QUENCH FLOW INSTRUMENT KIN-TEK RQF-3 VALVE/SYRINGE CHAMBER Biorad Laboratory Real Time PCR System& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Instrument Configuration: Assay Technology Adapta® & Setup for GeneBLAzer® Assay on Molecular Devices FilterMax™ F5 Microplate Reader with SoftMax® Pro 6. 3 Pages. Molecular Devices Multi-Mode Microplate Reader FilterMax F5. Page 154: Ordering Parts And Accessories. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. , Sunnyvale, CA, USA). Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. ] Example of document to evaluate if the reader . Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. com A. 3 Pages. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by normalizing to total protein. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Hit enter to search or ESC to close. 100 fmol 1536-well (8 µL) 0. Datapaq Express (Vial Rack Bottom Label 2D Reader) XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING. Run a variety of luminometric research applications with the Thermo Scientific Luminoskan microplate luminometer, a compact and robust instrument offering versatile plate formats, fast reading speeds and up to three dispensers. Plate chamber design of the SpectraMax category 1 microplate readers. SpectraMax iD5. A microplate consists of small wells wherein separate reactions take place. 09 CAD. Thereafter, the dye liquid was removed, the formazan was solubilized with dimethyl sulfoxide (DMSO) under continuous agitation for 15 min, and the absorbance of each well was read by the means of a microplate reader (FilterMax F5 Multimode Microplate Reader; Molecular Devices) at a wavelength of 570 µm. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. . MD, MPH, Milton J. 3. Align the two slots on the bottle holder with the two pegs on the rail. ” e. prt Read the plate and then save the experiment. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. Synergy HTX and Synergy LX. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. Add to my manuals. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. See More Information. 3 Pages. ; Page 2 For research use only. 425 or. The Molecular Devices FilterMax F5 Multi-Mode Plate Reader was tested for compatibility with Life Technologies’ Z'-LYTE® assays using the Z'-LYTE® Tyr6 kit (PV4122) against. 40266 Email: drugdiscoverytech@lifetech. Plate Formats:. FilterMax F3 & F5 Multi-Mode Microplate Readers from. Multi-Mode Microplate Reader. $3,000 USD. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. For 3D cell proliferation assay, (high concentrations) in 3D cellular proliferation assay, 20, 100 or 500 µg/ ml MG were used. searchPolymerization and polymerization kinetics were monitored in a Corning 96-well black flat bottom polystyrene microplate (Corning, Lowell, MA, USA) using a FilterMax F5 Multi-Mode Microplate Reader. FilterMax F3 & F5 Multi-Mode Microplate Readers: SpectraMax i3x Multi-Mode Microplate Reader: SpectraMax Paradigm Multi-Mode Microplate Detection System: Citations: Figures: Catalog Number: Inquire: FilterMax F3 / FilterMax F5: i3x: Inquire: Price: Description: The SpectraMax® i3 Multi-Mode Detection Platform comes with standard. 9). com A. FilterMax® F3 & F5 Multi-Mode Microplate Readers. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 6 reviews. Related Products: FilterMax F3 &. 2 Pages. The haemolytic activity was determined in the cell supernatant by optical density readings at 570 nm (FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices). FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. Page 24: Synergy™ Ht Multi-Mode Microplate Reader Internal plate shaking is also supported. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry. Owner, Zeckhausen. Supplier: Molecular Devices.